Journal: International Journal of Molecular Sciences

Article Title: Comparative Study of Nanoparticle Blood Circulation after Forced Clearance of Own Erythrocytes (Mononuclear Phagocyte System-Cytoblockade) or Administration of Cytotoxic Doxorubicin- or Clodronate-Loaded Liposomes

doi: 10.3390/ijms241310623

Figure Lengend Snippet: Characterization of magnetic particles.

Article Snippet: The following reagents were used in the experiments: L-α-phosphatidylcholine from egg yolk (EPC), cholesterol, sucrose, iron (III) chloride hexahydrate, iron (II) chloride tetrahydrate, carboxymethyl dextran (CMD) sodium salt, N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC), N-hydroxysulfosuccinimide sodium salt (sulfo-NHS), phosphate-buffered saline (PBS) tablets (Sigma-Aldrich, St Louis, MO, USA); 2-(N-morpholino)ethanesulfonic acid (MES) monohydrate, Tris hydrochloride, EDTA, Triton X-100 (AppliChem, Darmstadt, Germany); ammonium hydroxide, nitric acid (Component-reaktiv, Moscow, Russia); sulfo-Cyanine5 amine (Cy5), Cyanine7.5 amine (Cy7.5) (Lumiprobe, Moscow, Russia); HEPES (Dia-M, Moscow, Russia), dimethyl sulfoxide (DMSO) (Chimmed, Moscow, Russia), ammonium sulfate (Helicon, Moscow, Russia); mouse antibodies against mouse red blood cells (RBC) clone 34-3C (Hycult Biotech, Uden, Netherlands); glucose for intravenous infusion (Kraspharma, Moscow, Russia); Zoletil 100 (Virbac, Carros, France); Xyla (Interchemie Werken De Adelaar Eesti AS, Püünsi, Estonia); Estapor 200-nm carboxylated and Estapor 1000 nm tosyl-activated polystyrene magnetic beads (Merck Millipore, Billerica, MA, USA); fluidMAG-CMX carboxymethyl dextran-coated magnetic beads (Chemicell, Berlin, Germany); SMOFlipid (Fresenius Kabi, Bad Homburg, Germany); doxorubicin-Teva (Pharmachemie, Haarlem, Netherlands); and clodronic acid (60 mg/mL, Bonefos ® , Bayer, Leverkusen, North Rhine-Westphalia, Germany).

Techniques: Magnetic Beads